作者
Anique D Ter Braake, Coby Eelderink, Lara W Zeper, Andreas Pasch, Stephan JL Bakker, Martin H de Borst, Joost GJ Hoenderop, Jeroen HF de Baaij
发表日期
2020/5/1
期刊
Nephrology Dialysis Transplantation
卷号
35
期号
5
页码范围
765-773
出版商
Oxford University Press
简介
Background
Phosphate (Pi) toxicity is a strong determinant of vascular calcification development in chronic kidney disease (CKD). Magnesium (Mg2+) may improve cardiovascular risk via vascular calcification. The mechanism by which Mg2+ counteracts vascular calcification remains incompletely described. Here we investigated the effects of Mg2+ on Pi and secondary crystalline calciprotein particles (CPP2)-induced calcification and crystal maturation.
Methods
Vascular smooth muscle cells (VSMCs) were treated with high Pi or CPP2 and supplemented with Mg2+ to study cellular calcification. The effect of Mg2+ on CPP maturation, morphology and composition was studied by medium absorbance, electron microscopy and energy dispersive spectroscopy. To translate our findings to CKD patients, the effects of Mg2+ on calcification propensity (T50) were measured in sera from …
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AD Ter Braake, C Eelderink, LW Zeper, A Pasch… - Nephrology Dialysis Transplantation, 2020