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Giot1 is a kruppel-type zinc finger protein induced by gonadotropin in teca cells of the ovary and also identified in the central nervous system areas related with hydroeletrolitic and energy homeostasis. Osmotic challenges induce simultaneously an increase in the Giot1 and AVP mRNA expressions in the paraventricular nucleus (PVN) of the hypothalamus. Our hypothesis was that Giot1 had a central role on hydromineral and energy balance modulating the ingestive behavior. To confirm this hypothesis, female Sprague-Dawley(200g) rats were maintained on metabolic cages with free access to diet and fluids. Water was offered during 8 days followed by water and 0.3M NaCl solution during 12 days. After these 20 days, animals were randomly assigned into the Giot1 gene knockdown group and submitted to a bilateral microinjection of small hairpin RNA expressing lentiviral vector (shGiot1) into the PVN …