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It has been shown that stromal–vascular fraction isolated from adipose tissues contains an abundance of CD34⁺ cells. Histological analysis of adipose tissue revealed that CD34⁺ cells are widely distributed among adipocytes and are predominantly associated with vascular structures. The majority of CD34⁺ cells from freshly isolated stromal–vascular fraction were CD31⁻/CD144⁻ and could be separated from a distinct population of CD34⁺/CD31⁺/CD144⁺ (endothelial) cells by differential attachment on uncoated plastic. The localization of CD34⁺ cells within adipose tissue suggested that the nonendothelial population of these cells occupied a pericytic position. Analysis of surface and intracellular markers of the freshly isolated CD34⁺/CD31⁻/CD144⁻ adipose-derived stromal cells (ASCs) showed that >90% coexpress mesenchymal (CD10, CD13, and CD90), pericytic (chondroitin sulfate proteoglycan, CD140a …