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The objective of the study was to observe the morphological changes on root canal dentin after Er:YAG laser and Nd:YAG laser treatment. Twenty-one teeth biomechanically prepared were divided into three groups with seven teeth of each. Group A was unlased as a control. In group B, Er:YAG laser and in group C, Nd:YAG laser was applied to the root canal dentin. The roots were split longitudinally and examined using a scanning electron microscopy for the evaluation of debris, smear layer, and recrystallization. There was no statistically significant difference between the groups. This study indicates that laser beam is not effective in removing debris and smear layer.