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To isolate pectinase producing bacterial strains from various samples and to investigate the co-production of pectinase and biosurfactant by this isolated strain.

Isolation, screening, selection and identification of pectinolytic bacteria. Production of pectinase and analysis of exo-pectinase types. Partial purification by ammonium sulphate precipitation and dialysis. The study also analyses the capability of the selected strain for biosurfactant production.

Department of Life Sciences, University of Calicut, Kerala - 673635, India, between July 2013 and November 2014.

The pectinolytic bacteria was isolated and screened by iodine plate assay method. The enzyme production was confirmed by 3,5-dinitrosalicylic acid (DNS) method. The potent enzyme producing strain was biochemically characterised and identified by 16S rRNA gene sequence analysis. Thiobarbituric acid (TBA) and DNS assay were performed to test the types of exo-pectinase produced. Partial purification of the enzyme was done by ammonium sulphate precipitation. Biosurfactant production by the strain was tested by methods like foam formation, drop collapse test, oil displacement test, microplate assay, hemolytic assay, penetration assay, emulsification activity and bacterial adhesion to hydrocarbon (BATH) assay.

Thirty six pectinolytic bacterial strains were screened from the collected samples. Four isolates were selected on the basis of zone size (20 mm to 26 mm) in well plate screening method. In which, the isolate showed maximum enzyme production in DNS assay (0.707 U/mL) is the same which exhibited …