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This study was undertaken as an effort to develop the knowledge that could provide information and clue regarding the algae niche in the natural environment. We utilized natural water medium to mimic natural water body in laboratory to grow algae, to understand the algae niche in the ecosystems and to demonstrate the potential of algal CO₂ bio-fixation. The continuously long-term operation in the laboratory demonstrated the impressed algal function as in nature. The study showed that natural water resource had sufficient nutrients for microalgae growth. We also found evidences to prove that 100% carbon source came from the atmospheric CO₂ absorption to reach the extremely high CO₂ consumption efficiency. This study illustrated that the growth conditions of algal niche in the natural CO₂ absorption processes is helpful in understanding the algae ecology in water bodies and is also valuable for future global …