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Malaysia is one of the world’s largest palm oil producers and exporters, which generates several million tons of palm oil mill effluent (POME). POME is discharged into the water sources and considered as a major environmental pollutants in Malaysia. Alternatively, POME also contains some nutrients and several minerals, thereby POME could prove as a suitable medium for microbial-based applications, such as wastewater treatment and biofuel production. Different techniques have been employed to effectively utilize the POME at point of generation. One of the alternatives is the cultivation of microalgae in the enriched medium of POME. The present study used POME as growth medium for cultivation of Chlorella sp. and determines the effect of light intensity (ranging from 900 to 12,000 lux) and different CO₂ concentrations (ranging from 5% (v/v) to 20% (v/v)) by both experimentally and design expert …