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A new simple, sensitive and selective liquid chromatography coupled with mass spectrometry (LC-MS) method for quantification of ciprofloxacin in human plasma was validated. Ciprofloxacin and ofloxacin, as internal standard, were analysed on a SB-C18 column (Agilent Technologies, 100 mm x 3 mm ID, 3.5 μm) under isocratic conditions using a mobile phase of a 70: 30 (v/v) mixture of 0.1%(v/v) formic acid in water and acetonitrile. The flow rate was 0.5 mL/min at the column temperature of 25 C. The detection of the analyte was in SIM mode using a triple quadrupole mass spectrometer with electrospray positive ionisation. The monitored ions were m/z 332 for ciprofloxacin and m/z for 362 for ofloxacin. The sample preparation was very simple and consisted of protein precipitation from 0.2 mL plasma using 0.4 mL of 0.05% acetic acid solution in methanol containing 0.5 mg/mL internal standard. Linear calibration …