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In the present study, ZnO, CuO, CuO/ZnO, 5% Ag/CuO, 10% Ag/CuO, 5%Ag/ZnO, and 10% Ag/ZnO NPs were synthesized to develop bio-nano medicines with potent antibacterial, antifungal, and antioxidant properties. XRD, HR-SEM, EDAX, and HR-TEM spectral analyses were used to establish the structural characteristics of the synthesized NPs. According to the XRD study, the average particle size for CuO NPs was 23.42 ​nm, for ZnO NPs it was 28.00 ​nm, and for CuO/ZnO nanocomposite; 25.58 ​nm. The agglomeration of NPs in ZnO and CuO NPs, as well as the presence of agglomeration and nanorods in the CuO–ZnO nanocomposite, were identified using HR-SEM. In the present study, CuO NPs have a cubic crystal structure, whereas ZnO NPs have a hexagonal crystal structure, as confirmed by HR-TEM. Both cubic and hexagonal crystal lattices were found in the CuO/ZnO nanocomposites. The Ag …