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A growth-inhibitory polysaccharide (GIPinv) was purified using size-exclusion and ion-exchange chromatography from the fourth sodium hydroxide extraction step of a fungus found in British Columbia. The fungus was genetically identified as a member of the Paxillus involutus complex. GIPinv has an average molecular weight of 229 kDa and is a heteroglycan composed of glucose (65.9%), galactose (20.8%), mannose (7.8%), fucose (3.2%) and xylose (2.3%). GC–MS methylation analysis suggests that GIPinv has mixed linkages in the backbone containing (1 → 6)-Gal (25.5%), (1 → 4)-Glc (18.3%), (1 → 6)-Glc (8.3%), (1 → 3)-Glc (5.3%) and (1 → 2)-Xyl (4.5%). GIPinv has branching points at (1 → 2, 6)-Man (8.6%) and (1 → 3, 6)-Man (4.9%) having unsubstituted fucose (8.3%) and glucose (16.3%) as terminal sugars. GIPinv had growth-inhibitory activity against several cancer cell lines and triggered apoptosis …