作者
Robert Geisler, Gerd-Jörg Rauch, Herwig Baier, Frauke Van Bebber, Linda Broβ, Marcus PS Dekens, Karin Finger, Cornelia Fricke, Michael A Gates, Horst Geiger, Silke Geiger-Rudolph, Darren Gilmour, Stefanie Glaser, Lara Gnügge, Hinrich Habeck, Katy Hingst, Scott Holley, Jeremy Keenan, Anette Kirn, Holger Knaut, Deval Lashkari, Florian Maderspacher, Ulrike Martyn, Stephan Neuhauss, Carl Neumann, Teresa Nicolson, Francisco Pelegri, Russell Ray, Jens M Rick, Henry Roehl, Tobias Roeser, Heike E Schauerte, Alexander F Schier, Ulrike Schönberger, Helia-Berrit Schönthaler, Stefan Schulte-Merker, Catrin Seydler, William S Talbot, Christian Weiler, Christiane Nüsslein-Volhard, Pascal Haffter
发表日期
1999/9
期刊
Nature genetics
卷号
23
期号
1
页码范围
86-89
出版商
Nature Publishing Group
简介
Recent large-scale mutagenesis screens have made the zebrafish the first vertebrate organism to allow a forward genetic approach to the discovery of developmental control genes 1, 2, 3. Mutations can be cloned positionally, or placed on a simple sequence length polymorphism (SSLP) map 4, 5, 6 to match them with mapped candidate genes and expressed sequence tags 7, 8 (ESTs). To facilitate the mapping of candidate genes and to increase the density of markers available for positional cloning, we have created a radiation hybrid (RH) map of the zebrafish genome. This technique is based on somatic cell hybrid lines produced by fusion of lethally irradiated cells of the species of interest with a rodent cell line. Random fragments of the donor chromosomes are integrated into recipient chromosomes or retained as separate minichromosomes 9, 10. The radiation-induced breakpoints can be used for mapping in …
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R Geisler, GJ Rauch, H Baier, F Van Bebber, L Broβ… - Nature genetics, 1999