作者
D Clark Files, Franco R D'Alessio, Laura F Johnston, Priya Kesari, Neil R Aggarwal, Brian T Garibaldi, Jason R Mock, Jessica L Simmers, Antonio DeGorordo, Jared Murdoch, Monte S Willis, Cam Patterson, Clarke G Tankersley, Maria L Messi, Chun Liu, Osvaldo Delbono, J David Furlow, Sue C Bodine, Ronald D Cohn, Landon S King, Michael T Crow
发表日期
2012/4/15
期刊
American journal of respiratory and critical care medicine
卷号
185
期号
8
页码范围
825-834
出版商
American Thoracic Society
简介
Rationale: Acute lung injury (ALI) is a debilitating condition associated with severe skeletal muscle weakness that persists in humans long after lung injury has resolved. The molecular mechanisms underlying this condition are unknown.
Objectives: To identify the muscle-specific molecular mechanisms responsible for muscle wasting in a mouse model of ALI.
Methods: Changes in skeletal muscle weight, fiber size, in vivo contractile performance, and expression of mRNAs and proteins encoding muscle atrophy–associated genes for muscle ring finger-1 (MuRF1) and atrogin1 were measured. Genetic inactivation of MuRF1 or electroporation-mediated transduction of miRNA-based short hairpin RNAs targeting either MuRF1 or atrogin1 were used to identify their role in ALI-associated skeletal muscle wasting.
Measurements and Main Results: Mice with ALI developed profound muscle atrophy and preferential loss of …
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DC Files, FR D'Alessio, LF Johnston, P Kesari… - American journal of respiratory and critical care …, 2012