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Assessing the current levels of greenhouse gas (GHG) emissions from road transportation projects allows for benchmarking and is essential for potential emissions reduction. The objective of this study was to estimate the GHG emissions associated with the construction and operation of three road cases—two primary roads and one secondary road network—in Abu Dhabi, United Arab Emirates. The GHG emissions produced by the study cases were estimated using the RoadCO₂ estimation tool. Results showed that the total emissions (in kg CO₂e/m²/y) range from 76 for the secondary road case to 1100 for the primary road cases. The operation phase is responsible for 94–98% of these emissions; the construction phase is responsible for the rest. Road works contributed the most to GHG emissions during the construction phase. The contribution of the remaining categories of the construction phase fluctuates within a certain case and among the considered cases. The equipment used in the construction phase for the three cases contributed 15–70% of the total phase emissions, while the remaining emissions were due to construction materials. In the operation phase, emissions were mainly generated by vehicle movement. Street lighting also contributed to emissions during the operation phase. On the other hand, the irrigation of planted trees along the road had a very low impact on GHG emissions, and carbon sequestration by these trees had a negligible effect in terms of acting as a carbon sink. The results obtained from this study were compared with other cases reported in the literature.