作者
Wilson LW Tan, Benson TS Lim, Chukwuemeka GO Anene-Nzelu, Matthew Ackers-Johnson, Albert Dashi, Kelvin See, Zenia Tiang, Dominic Paul Lee, Wee Woon Chua, Tuan DA Luu, Peter YQ Li, Arthur Mark Richards, Roger SY Foo
发表日期
2017/3/1
期刊
Cardiovascular research
卷号
113
期号
3
页码范围
298-309
出版商
Oxford University Press
简介
Aims
Circular RNA (circRNA) is a newly validated class of single-stranded RNA, ubiquitously expressed in mammalian tissues and possessing key functions including acting as microRNA sponges and as transcriptional regulators by binding to RNA-binding proteins. While independent studies confirm the expression of circRNA in various tissue types, genome-wide circRNA expression in the heart has yet to be described in detail.
Methods and results
We performed deep RNA-sequencing on ribosomal-depleted RNA isolated from 12 human hearts, 25 mouse hearts and across a 28-day differentiation time-course of human embryonic stem cell-derived cardiomyocytes. Using purpose-designed bioinformatics tools, we uncovered a total of 15 318 and 3017 cardiac circRNA within human and mouse, respectively. Their abundance generally correlates with the abundance of their …
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WLW Tan, BTS Lim, CGO Anene-Nzelu… - Cardiovascular research, 2017