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An aqueous solution of carboxylmethylcellulose with phenolic hydroxyl groups (CMC-Ph) is gellable within 1min via a peroxidase-catalyzed oxidative reaction under mild conditions suitable for mammalian cells. In this research, we evaluated cellular adhesion and proliferation on the resultant hydrogel, and the feasibility of the hydrogel as a substrate for cell sheet technology. Within 4h of seeding, 76.9% of L929 fibroblast cells adhered to the gel and showed similar morphology of spreading to that on cell culture dish. Subsequently, the adherent cells proliferated on the gel and formed a confluent monolayer after 168h of culture. From the confluent monolayer we could harvest a cell sheet after about 5min of digestion of the gel using cellulase dissolved in medium at 5Uml⁻¹. The cells in the cell sheet showed well-preserved morphology similar to that shown before they were detached from the gel. In addition, the …