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Cell-enclosing spherical vehicles have been studied as effective tools for the treatment of diseases, 1, 2 production of antibodies, 3 and drug screening. 4 Recently, this type of device has also attracted attention as a novel tool for tissue engineering. 5, 6 From a structural viewpoint, the spherical vehicles can be classified into two categories:(1) vehicles with a solid core, designated “microparticles,” and (2) vehicles with a liquid core, designated “microcapsules.” The latter vehicles are more suitable for the interior formation of spherical tissues. Spherical tissues are increasingly recognized as valuable tools in tissue engineering because they appear to mimic the morphology and physiology of the cells in living tissues and organs better than conventional, two-dimensional monolayer cultures. 7 One problem that may occur when developing spherical tissues from individual cells in vitro is the formation of necrotic regions …