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BSA was entrapped in particles of different sizes (200, 500 and 1000nm) prepared from poly(d,l-lactic-co-glycolic) acid by a double emulsion method. The particles were given, either intranasally, orally or subcutaneously, to Balb/c mice and the serum IgG, IgG1 and IgG2a response elicited was compared to that obtained by the subcutaneous administration of either free antigen, free antigen emulsified 1:1 with Freund’s Complete Adjuvant (FCA), or free antigen administered with Al(OH)₃. The administration of 1000nm particles generally elicited a higher serum IgG response than that obtained with the administration of 500 or 200nm sized nanospheres, the immune response for 500nm particles being similar than that obtained with 200nm by the subcutaneous and the oral route, and higher by the intranasal route. PLGA nanoparticles can elicit serum IgG2a responses by the three routes studied. No significant …