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Because of increase in antibiotic resistance, finding alternative treatments for controlling infections in oral cavity is critical. In this study we aimed to compare use of halite with CO2 laser radiation for controlling infections by S. aureus and P. aeruginosa. Staphilococcus aureus (ATCC 29213) and Pseudomonas aeruginosa (ATCC 27853) were used as standard strains. The effect of CO2 Laser was evaluated 5, 10, and 15 seconds after exposure to the standard suspension of bacterium with energy density of 12.5 J/cm2 at a distance of 17mm. halite (Chlorhexidine digluconate 0.05%, Cetylpyridinium chloride (CPC) 0.05% and Zinc lactate 0.14%) was examined in the same condition. The average number of microbes was lower in the Halita group than CO2 laser group before 15 second (P-value< 0.001). But after 15 second, No growth observed in CO2 laser group in contrary with Halita group (P-value and lt; 0.001). Average time for complete infection removal for Halita was 60 second and for CO2 laser was 15 seconds. findings of the present study showed that CO2 laser radiation is valuable tools for infection control in oral cavity infections. Also halite was successful for infection remove after 60 seconds. Using CO2 laser radiation in combination of halita mouthwash can help for complete eradication of infections from oral cavity.