作者
HTT Phan, N Stoesser, IE Maciuca, F Toma, E Szekely, M Flonta, ATM Hubbard, L Pankhurst, T Do, TEA Peto, AS Walker, DW Crook, D Timofte
发表日期
2018/3/1
期刊
Journal of Antimicrobial Chemotherapy
卷号
73
期号
3
页码范围
672-679
出版商
Oxford University Press
简介
Background and Objectives
Serratia marcescens is an emerging nosocomial pathogen, and the carbapenemase blaNDM has been reported in several surveys in Romania. We aimed to investigate the molecular epidemiology of S. marcescens in two Romanian hospitals over 2010–15, including a neonatal NDM-1 S. marcescens outbreak.
Methods
Isolates were sequenced using Illumina technology together with carbapenem-non-susceptible NDM-1-positive and NDM-1-negative Klebsiella pneumoniae and Enterobacter cloacae to provide genomic context. A subset was sequenced with MinION to fully resolve NDM-1 plasmid structures. Resistance genes, plasmid replicons and ISs were identified in silico for all isolates; an annotated phylogeny was reconstructed for S. marcescens. Fully resolved study NDM-1 plasmid sequences were compared with the most closely …
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HTT Phan, N Stoesser, IE Maciuca, F Toma, E Szekely… - Journal of Antimicrobial Chemotherapy, 2018