作者
Lasse Skibsbye, Claire Poulet, Jonas Goldin Diness, Bo Hjorth Bentzen, Lei Yuan, Utz Kappert, Klaus Matschke, Erich Wettwer, Ursula Ravens, Morten Grunnet, Torsten Christ, Thomas Jespersen
发表日期
2014/7/1
期刊
Cardiovascular research
卷号
103
期号
1
页码范围
156-167
出版商
Oxford University Press
简介
Aims
Small-conductance calcium-activated potassium (SK) channels are expressed in the heart of various species, including humans. The aim of the present study was to address whether SK channels play a functional role in human atria.
Methods and results
Quantitative real-time PCR analyses showed higher transcript levels of SK2 and SK3 than that of the SK1 subtype in human atrial tissue. SK2 and SK3 were reduced in chronic atrial fibrillation (AF) compared with sinus rhythm (SR) patients. Immunohistochemistry using confocal microscopy revealed widespread expression of SK2 in atrial myocytes. Two SK channel inhibitors (NS8593 and ICAGEN) were tested in heterologous expression systems revealing ICAGEN as being highly selective for SK channels, while NS8593 showed less selectivity for these channels. In isolated atrial myocytes from SR patients, both …
学术搜索中的文章
L Skibsbye, C Poulet, JG Diness, BH Bentzen, L Yuan… - Cardiovascular research, 2014