作者
Subhodip Adhicary, Kayleigh Fanelli, Sera Nakisli, Brittney Ward, Isaac Pearce, Corinne M Nielsen
发表日期
2023/6
期刊
Stroke
卷号
54
期号
6
页码范围
1593
出版商
NIH Public Access
简介
Background:
Brain arteriovenous malformations (bAVM) are characterized by enlarged blood vessels, which direct blood through arteriovenous (AV) shunts, bypassing the artery-capillary-vein network and disrupting blood flow. Clinically, bAVM treatments are invasive and not routinely applicable. There is critical need to understand mechanisms of bAVM pathologies and develop pharmacological therapies.
Methods:
We used an in vivo mouse model of Rbpj-mediated bAVM, which develops pathologies in the early postnatal period and an siRNA in vitro system to knockdown RBPJ in human brain microvascular endothelial cells (ECs). To understand molecular events regulated by endothelial Rbpj, we conducted RNA-Seq and ChIP-Seq analyses from isolated brain ECs.
Results:
Rbpj-deficient (mutant) brain ECs acquired abnormally rounded shape (with no change to cell area), altered basement membrane …
学术搜索中的文章
S Adhicary, K Fanelli, S Nakisli, B Ward, I Pearce… - Stroke, 2023