作者
Haidy El‐Azzamy, Svetlana V Dambaeva, Dimantha Katukurundage, Maria D Salazar Garcia, Annie Skariah, Youssef Hussein, Alfredo Germain, Emilio Fernandez, Alice Gilman‐Sachs, Kenneth D Beaman, Joanne Kwak‐Kim
发表日期
2018/10
期刊
American journal of reproductive immunology
卷号
80
期号
4
页码范围
e13024
简介
Problem
Angiogenesis and vascular remodeling in secretory endometrium represent one of the crucial steps in pregnancy establishment, for which uterine NK (uNK) cells have an important role. Impairment of these steps may proceed to implantation and instigate initial pathology of recurrent pregnancy losses (RPL). In this study, we aim to investigate vascular development and density of uNK cells in secretory endometrium of women with RPL.
Methods of study
Mid‐secretory phase endometrial tissues from women with RPL (n = 15) and fertile controls (n = 7) were investigated. CD56+ and CD16+ uNK cells, CD31+ vascular endothelial cells and smooth muscle myosin (SMM)+. Vascular smooth muscle cells (VSMC) expressing SMM were investigated using immunohistochemistry and western blot. High‐throughput quantitative real‐time polymerase chain reaction (qRT‐PCR) was used as well.
Results
CD56 …
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H El‐Azzamy, SV Dambaeva, D Katukurundage… - American journal of reproductive immunology, 2018