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The aim of the study was to isolate and characterize potential autochthonous bacteria for biomineralization of Pb in mine tailings. A total of four bacteria were isolated from the soil samples and assayed for tolerance to Pb and other heavy metals. Isolate KK1 exhibited maximum Pb resistance and was subsequently identified as Bacillus sp. based on the partial 16S rRNA gene sequences. The isolate KK1 reduced the Pb ions and did not harbor pbrT gene. Selective sequential extraction of bioaugmented soil revealed that the isolate significantly reduced (26%) the exchangeable fraction and increased (38%) the carbonate fraction of Pb. X-ray diffraction studies confirmed the role of bacterially induced calcite precipitation in the bioremediation of mine tailings. A significant increase in the urease (334%), DHO (dehydrogenase) (14%), and phosphatase (37%) activity was observed in the bioaugmented mine soil.