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The present study reports an eco-friendly, rapid and easy method for synthesis of silver nanoparticles (AgNPs) using Tinospora cordifolia as a reducing and capping agent. The different factor such as silver nitrate (AgNO₃) concentration, fresh weight of T. cordifolia leaf, incubation time, and pH affecting silver reduction was investigated using Response surface methodology based Box–Behnken design (BBD). The optimum conditions were AgNO₃ (1.25 mM), incubation time (15 h), Temperature (45 °C) and pH (4.5). T. cordifolia leaf extract can reduces silver ions into AgNPs within 30 min after heating the reaction mixture (60 °C) as indicated by the developed reddish brown color. The UV-Vis spectrum of AgNPs revealed a characteristic surface plasmon resonance (SPR) peak at 430 nm. AgNPs were characterized X-ray diffraction (XRD) revealed their crystalline nature and their average size of nanoparticles was 30 …