作者
Timothy H Bayburt, Andrew J Leitz, Guifu Xie, Daniel D Oprian, Stephen G Sligar
发表日期
2007/5/18
期刊
Journal of biological chemistry
卷号
282
期号
20
页码范围
14875-14881
出版商
Elsevier
简介
Nanodiscs are nanometer scale planar membranes of controlled size that are rendered soluble in aqueous solution via an encircling amphipathic membrane scaffold protein "belt" (Bayburt, T. H., Grinkova, Y. V., and Sligar, S. G. (2002) Nano. Lett. 2, 853–856). Integral membrane proteins can be self-assembled into the Nanodisc bilayer with defined stoichiometry, which allows an unprecedented opportunity to investigate the nature of the oligomerization state of a G-protein-coupled receptor and its coupling to heterotrimeric G-proteins. We generated Nanodiscs having one and two rhodopsins present in the 10-nm-diameter lipid bilayer domain. Efficient transducin activation and isolation of a high affinity transducin-metarhodopsin II complex was demonstrated for a monodisperse and monomeric receptor. A population of Nanodiscs containing two rhodopsins was generated using an increased ratio of receptor to …
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TH Bayburt, AJ Leitz, G Xie, DD Oprian, SG Sligar - Journal of biological chemistry, 2007