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The inflammatory response to parasites, bacteria, and viruses is mediated by multiple host factors. TNF-α is one of the most pleiotropic cytokines in mammals, but has yet to be identified in avian species. In the current study, we isolated a full-length cDNA encoding the chicken homologue of LPS-induced TNF-α factor (LITAF), transcription factor, with an open reading frame of 148 amino acids and a predicted molecular mass of 16.0kDa. Quantitative RT-PCR analysis showed that chicken LITAF mRNA was predominantly expressed in spleen and intestinal intraepithelial lymphocytes (IELs). LITAF mRNA levels were up-regulated following in vitro stimulation of macrophages for 4h with Escherichia coli or Salmonella typhimurium endotoxin, and 18–48h after treatment with Eimeria acervulina, E. maxima, or E. tenella, three causative agents of avian coccidiosis. LITAF mRNA was up-regulated by more than 700-fold in …