作者
Maria L Kuzmina, Thomas WA Braukmann, Aron J Fazekas, Sean W Graham, Stephanie L Dewaard, Anuar Rodrigues, Bruce A Bennett, Timothy A Dickinson, Jeffery M Saarela, Paul M Catling, Steven G Newmaster, Diana M Percy, Erin Fenneman, Aurélien Lauron‐Moreau, Bruce Ford, Lynn Gillespie, Ragupathy Subramanyam, Jeannette Whitton, Linda Jennings, Deborah Metsger, Connor P Warne, Allison Brown, Elizabeth Sears, Jeremy R Dewaard, Evgeny V Zakharov, Paul DN Hebert
发表日期
2017/12
期刊
Applications in Plant Sciences
卷号
5
期号
12
页码范围
1700079
出版商
Botanical Society of America
简介
- Premise of the study: Constructing complete, accurate plant DNA barcode reference libraries can be logistically challenging for large‐scale floras. Here we demonstrate the promise and challenges of using herbarium collections for building a DNA barcode reference library for the vascular plant flora of Canada.
- Methods: Our study examined 20,816 specimens representing 5076 of 5190 vascular plant species in Canada (98%). For 98% of the specimens, at least one of the DNA barcode regions was recovered from the plastid loci rbcL and matK and from the nuclear ITS2 region. We used beta regression to quantify the effects of age, type of preservation, and taxonomic affiliation (family) on DNA sequence recovery.
- Results: Specimen age and method of preservation had significant effects on sequence recovery for all markers, but influenced some families more (e.g., Boraginaceae) than others (e.g., Asteraceae …
学术搜索中的文章
ML Kuzmina, TWA Braukmann, AJ Fazekas… - Applications in Plant Sciences, 2017