作者
Karel Bezstarosti, Alireza Ghamari, Frank G Grosveld, Jeroen AA Demmers
发表日期
2010/9/3
期刊
Journal of proteome research
卷号
9
期号
9
页码范围
4464-4475
出版商
American Chemical Society
简介
Although enzyme catalyzed 18O labeling has been used as a tool in quantitative proteomics, this type of labeling has not yielded the same impact yet as alternative techniques for quantitation like SILAC or labeling with chemical mass tags. The practical difficulties involved in 18O labeling, most importantly the occurrence of incomplete labeling and, as a result, the difficulties in data analysis and interpretation have hampered its implementation in high-throughput comparative proteomics protocols. In this paper, we have optimized the 18O labeling procedure to such an extent that complete labeling can be achieved in a routine manner. We have implemented this approach into a protein−protein interaction analysis pipeline to differentiate between bona fide interaction partners of the low-level expressing cell cycle regulator cyclin-dependent kinase 9 (Cdk9) and nonspecifically binding or background proteins …
学术搜索中的文章
Differential proteomics based on 18O labeling to determine the cyclin dependent kinase 9 interactome
K Bezstarosti, A Ghamari, FG Grosveld, JAA Demmers - Journal of proteome research, 2010