作者
Shifei Qin, Chunfen Fan, Xiaohong Li, Yi Li, Jian Hu, Chaofeng Li, Keming Luo
发表日期
2020/12
期刊
Biotechnology for biofuels
卷号
13
页码范围
1-14
出版商
BioMed Central
简介
Background
The recalcitrance of lignocellulosic biomass provided technical and economic challenges in the current biomass conversion processes. Lignin is considered as a crucial recalcitrance component in biomass utilization. An in-depth understanding of lignin biosynthesis can provide clues to overcoming the recalcitrance. Laccases are believed to play a role in the oxidation of lignin monomers, leading to the formation of higher-order lignin. In plants, functions of only a few laccases have been evaluated, so little is known about the effect of laccases on cell wall structure and biomass saccharification.
Results
In this study, we screened a gain-of-function mutant with a significant increase in lignin content from Arabidopsis mutant lines overexpressing a full-length poplar cDNA library. Further analysis confirmed that a Chinese white poplar (Populus …
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