作者
Jeoung Soo Lee, Dina M Basalyga, Agneta Simionescu, Jason C Isenburg, Dan T Simionescu, Narendra R Vyavahare
发表日期
2006/2/1
期刊
The American journal of pathology
卷号
168
期号
2
页码范围
490-498
出版商
Elsevier
简介
Calcification of vascular elastin occurs in patients with arteriosclerosis, renal failure, diabetes, and vascular graft implants. We hypothesized that pathological elastin calcification is related to degenerative and osteogenic mechanisms. To test this hypothesis, the temporal expression of genes and proteins associated with elastin degradation and osteogenesis was examined in the rat subdermal calcification model by quantitative real-time reverse transcription-polymerase chain reaction and specific protein assays. Purified elastin implanted subdermally in juvenile rats exhibited progressive calcification in a time-dependent manner along with fibroblast and macrophage infiltration. Reverse transcription-polymerase chain reaction analysis showed that relative gene expression levels of matrix metalloproteinases (MMP-2and MMP-9) and transforming growth factor-β1 were increased in parallel with calcification. Gelatin …
引用总数
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JS Lee, DM Basalyga, A Simionescu, JC Isenburg… - The American journal of pathology, 2006