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Factorial designs and second order response surface methodology (RSM) for medium optimization were employed for the growth of recombinant Escherichia coli cells carrying a plasmid encoding TaqI endonuclease as a part of the fermentation strategy for general recombinant protein production. The method used was effective in screening for nutritional requirements using limited number of experiments. The concentrations of carbon source (glucose), inorganic nitrogen ((NH₄)₂HPO₄), potassium (KH₂PO₄) and magnesium (MgSO₄·7H₂O) sources in medium were changed according to the central composite rotatable design consisting of 29 experiments, and the biomass yield was calculated. The optimum medium composition was found to be 15gL⁻¹ glucose, 6.6gL⁻¹ (NH₄)₂HPO₄, 20.1gL⁻¹ KH₂PO₄ and 1.7gL⁻¹ MgSO₄·7H₂O. The model prediction of 2.72gDCWL⁻¹ biomass at optimum conditions was …