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Combination of conservation agricultural practices such as reduced tillage and complex cropping systems can improve soil quality. However, the different effects of conservation practices on soil physicochemical and microbiological parameters need to be monitored, since distinct managements, crops, regions and soil types can lead to different responses. In addition, seasonality can also affect these parameters, leading to changes associated with the environmental conditions, such as temperature and moisture. To better detect differences in soil quality between agricultural practices, the most appropriate season of sampling must be identified. Therefore, the objective of this study was to assess the changes in soil physicochemical and microbiological parameters between four different agricultural practices from a 7-year experiment along two seasons with contrasting soil water content. We analyzed the differences …