作者
Sophie Tartare-Deckert, Carine Chavey, Marie-Noëlle Monthouel, Nadine Gautier, Emmanuel Van Obberghen
发表日期
2001/6/22
期刊
Journal of Biological Chemistry
卷号
276
期号
25
页码范围
22231-22237
出版商
Elsevier
简介
Alterations in the expression level of genes may contribute to the development and pathophysiology of obesity. To find genes differentially expressed in adipose tissue during obesity, we performed suppression subtractive hybridization on epididymal fat mRNA from goldthioglucose (GTG) obese mice and from their lean littermates. We identified the secreted proteinacidic and rich in cysteine (SPARC), a protein that mediates cell-matrix interactions and plays a role in modulation of cell adhesion, differentiation, and angiogenesis. SPARC mRNA expression in adipose tissue was markedly increased (between 3- and 6-fold) in three different models of obesity,i.e. GTG mice, ob/ob mice, and AKR mice, after 6 weeks of a high fat diet. Immunoblotting of adipocyte extracts revealed a similar increase in protein level. Using a SPARC-specific ELISA, we demonstrated that SPARC is secreted by isolated adipocytes. We found …
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S Tartare-Deckert, C Chavey, MN Monthouel, N Gautier… - Journal of Biological Chemistry, 2001