作者
Marie-Anne Germain, Laurent Chatel-Chaix, Bridget Gagne, Eric Bonneil, Pierre Thibault, Fabrine Pradezynski, Benoît de Chassey, Laurene Meyniel-Schicklin, Vincent Lotteau, Martin Baril, Daniel Lamarre
发表日期
2014/1/1
期刊
Molecular & Cellular Proteomics
卷号
13
期号
1
页码范围
184-203
出版商
Elsevier
简介
More than 170 million people worldwide are infected with the hepatitis C virus (HCV), for which future therapies are expected to rely upon a combination of oral antivirals. For a rapidly evolving virus like HCV, host-targeting antivirals are an attractive option. To decipher the role of novel HCV–host interactions, we used a proteomics approach combining immunoprecipitation of viral–host protein complexes coupled to mass spectrometry identification and functional genomics RNA interference screening of HCV partners. Here, we report the proteomics analyses of protein complexes associated with Core, NS2, NS3/4A, NS4B, NS5A, and NS5B proteins. We identified a stringent set of 98 human proteins interacting specifically with one of the viral proteins. The overlap with previous virus–host interaction studies demonstrates 24.5% shared HCV interactors overall (24/98), illustrating the reliability of the approach. The …
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