作者
Cecile L Maire, Marceline M Fuh, Kerstin Kaulich, Krystian D Fita, Ines Stevic, Dieter H Heiland, Joshua A Welsh, Jennifer C Jones, André Görgens, Tammo Ricklefs, Lasse Dührsen, Thomas Sauvigny, Simon A Joosse, Guido Reifenberger, Klaus Pantel, Markus Glatzel, Andras G Miklosi, James H Felce, Marco Caselli, Valerio Pereno, Rudolph Reimer, Hartmut Schlüter, Manfred Westphal, Ulrich Schüller, Katrin Lamszus, Franz L Ricklefs
发表日期
2021/7/1
期刊
Neuro-oncology
卷号
23
期号
7
页码范围
1087-1099
出版商
Oxford University Press
简介
Background
Genome-wide DNA methylation profiling has recently been developed into a tool that allows tumor classification in central nervous system tumors. Extracellular vesicles (EVs) are released by tumor cells and contain high molecular weight DNA, rendering EVs a potential biomarker source to identify tumor subgroups, stratify patients and monitor therapy by liquid biopsy. We investigated whether the DNA in glioblastoma cell-derived EVs reflects genome-wide tumor methylation and mutational profiles and allows noninvasive tumor subtype classification.
Methods
DNA was isolated from EVs secreted by glioblastoma cells as well as from matching cultured cells and tumors. EV-DNA was localized and quantified by direct stochastic optical reconstruction microscopy. Methylation and copy number profiling was performed using 850k arrays. Mutations were identified by …
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CL Maire, MM Fuh, K Kaulich, KD Fita, I Stevic… - Neuro-oncology, 2021