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This paper presents the sweetness identification of sugar solution using microwave technique. First, the dielectric constant and dielectric loss factor of sugar solution with different sugar concentration were studied. The dielectric properties of sugar solution at varied frequencies from 0.5 to 4 GHz were measured by open-ended coaxial probe method. The measured results were used to calculate the others microwave properties such as loss tangent and penetration depth. Second, the sweetness of sugar solution was identified by the measured transmission microwave signal sending through the sugar solution. The difference of transmission signal can be investigated with the different sweetness. Moreover, this proposed technique can predict the sweetness of unknown sugar solution with the good accuracy.