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Recently, the phyco-synthesis of nanoparticles has been applied as a reliable approach to modern research field, and it has yielded a wide spectrum of diverse uses in fields such as biological science and environmental science. This study used marine natural resource seaweed Sargassum myriocystum due to their unique phytochemicals and their significant attributes in giving effective response on various biomedical applications. The response is created by their stress-tolerant environmental adaptations. This inspired us to make an attempt using the above-mentioned charactersitics. Therfore, the current study performed phycosynthesis of titanium dioxide nanoparticles (TiO₂-NPs) utilising aqueous extracts of S. myriocystum. The TiO₂-NPs formation was confirmed in earlier UV–visible spectroscopy analysis. The crystalline structure, functional groups (phycomolecules), particle morphology (cubic, square, and …