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Biofilms are the syntrophic consortia of microbial sessile colonies that remain adhered to the biotic and abiotic surface with the help of self-secreted polymeric substances known as extracellular polymeric substances (EPS). The biofilms developed by the group of Gram-positive bacteria help them to survive within extremes of environmental conditions. This paper, insilico studies were performed on biofilm forming proteins to study the role of various phytocompounds in eradication of biofilm formationby Staphylococcus aureus. It was observed that nimbin showed maximum interaction with the biofilm forming proteins of S. aureus .