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1129 MORANGE2, A GENETICALLY ENCODED, PH SENSITIVE FLUORESCENT PROTEIN, IS AN ALTERNATIVE TO BCECF-AM TO MEASURE INTRACELLULAR PH TO DETERMNE NHE3 AND DRA ACTIVITY Rafiquel Sarker, Ming Tse, Ruxian Lin, George McNamara, Varsha Singh, Mark Donowitz Background: NHE3 (Na+/H+ exchanger3) and SLC26A3 (Cl-/HCO3-exchanger, DRA) are the major components of the intestinal neutral NaCl absorptive process and based on the intestinal segment, contribute to HCO 3-absorption and HCO 3-secretion. NHE3 and DRA are highly regulated by changes in second messengers, cAMP, cGMP and Ca 2+. Precise and convenient measurement of exchanger activity is necessary to allow rapid study of physiologic and pharmacologic functions. Some epithelial cells are difficult to load with AM ester dyes and loading may not be uniform. Methods: The use of a genetically …