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The affinities (K a) and association rate constants (k on) of 23 mouse (BALB/c) anti-lysozyme mAbs obtained after short and prolonged immunizations have been measured by plasmon resonance techniques. The affinities for the 23 Abs, measured using their Fab, range from K a= 1.1× 10 7 to 1.4× 10 10 M− 1. There is no significant correlation between time or dose of immunization and affinity or association rates, indicating no time-or dose-dependent maturation of the response within the doses and times that were explored. IgMs are produced early and late in the response, with intrinsic affinities< 10 5 M− 1. Two independently derived mAbs, D44. 1 (short term) and F10. 6.6 (from a longer term response), result from identical or nearly identical somatic recombination events of germline gene segments. F10. 6.6 has more mutations and a higher affinity constant (K a= 1.4× 10 10 M− 1) than D44. 1 (K a= 1.1× 10 7 M− 1 …