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Secreted protein, acidic and rich in cysteines (SPARC) is a secreted protein associated with increased aggressiveness of different human cancer types. In order to identify downstream mediators of SPARC activity, we performed a 2‐DE proteomic analysis of human melanoma cells following antisense‐mediated downregulation of SPARC expression. We found 23/504 differential spots, 15 of which were identified by peptide fingerprinting analysis. Three of the differential proteins (N‐cadherin (N‐CAD), clusterin (CLU), and HSP27) were validated by immunoblotting, confirming decreased levels of N‐CAD and CLU and increased amounts of HSP27 in conditioned media of cells with diminished SPARC expression. Furthermore, transient knock down of SPARC expression in melanoma cells following adenoviral‐mediated transfer of antisense RNA confirmed these changes. We next developed two different RNAs …