作者
Olga Cela, Claudia Piccoli, Rosella Scrima, Giovanni Quarato, Alessandra Marolla, Gilda Cinnella, Michele Dambrosio, Nazzareno Capitanio
发表日期
2010/8/1
期刊
Mitochondrion
卷号
10
期号
5
页码范围
487-496
出版商
Elsevier
简介
This study aimed to validate, in situ, proposed mechanisms of bupivacaine citotoxicity pointing to impairment of the mitochondrial oxidative metabolism. High resolution oxymetry, carried out on a panel of cell cultures, revealed a dual dose- and time-dependent effect of bupivacaine consisting of uncoupling of the mtΔμH+-controlled respiratory rates in a cyclosporine A-insensitive manner and further inhibition of the respiratory rates. Intriguingly, a relatively small decrease on the mtΔΨ (about 20mV) was sufficient to account for both the bupivacaine- and the FCCP-mediated impairment of the oxidative phosphorylation coupling thereby supporting a common protonophoric mechanism of action. The bupivacaine-induced depression of the cell respiration related to specific inhibition of complexes I and III and accompanied with production of reactive oxygen species. Importantly, inhibition of the respiratory chain …
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O Cela, C Piccoli, R Scrima, G Quarato, A Marolla… - Mitochondrion, 2010