查看文章

We used three different murine mammary carcinoma, each syngeneic in Balb/c mice, to study the early anti-tumor immune response. Initial analysis showed that the number of cells at the tumor sites almost doubled between 12–72 hours going from about 3e5 cells at 12 hours to about 6e5 cells at 72 hours. Surface staining revealed that the immune response during this time period was predominated by Gr1+ and F4/80+ cells. Strikingly, similar cell numbers and cell types were recruited to saline injected sites suggesting that these cells may be recruited non-specifically. However, closer examination revealed significant differences not only between the saline and tumor injected sites, but also among different tumor sites. For instance, at 12 hours post injection there were significantly more Gr1+ cells at the EMT6 tumor site than at the 4T1 tumor site, and at 48 hours there were significantly more Gr1+ cells at the 168 …