作者
Ashish Lal, Hyeon Ho Kim, Kotb Abdelmohsen, Yuki Kuwano, Rudolf Pullmann Jr, Subramanya Srikantan, Ramesh Subrahmanyam, Jennifer L Martindale, Xiaoling Yang, Fariyal Ahmed, Francisco Navarro, Derek Dykxhoorn, Judy Lieberman, Myriam Gorospe
发表日期
2008/3/26
期刊
PloS one
卷号
3
期号
3
页码范围
e1864
出版商
Public Library of Science
简介
Background
Expression of the tumor suppressor p16INK4a increases during aging and replicative senescence.
Methodology/Principal Findings
Here, we report that the microRNA miR-24 suppresses p16 expression in human diploid fibroblasts and cervical carcinoma cells. Increased p16 expression with replicative senescence was associated with decreased levels of miR-24, a microRNA that was predicted to associate with the p16 mRNA coding and 3′-untranslated regions. Ectopic miR-24 overexpression reduced p16 protein but not p16 mRNA levels. Conversely, introduction of antisense (AS)-miR-24 blocked miR-24 expression and markedly enhanced p16 protein levels, p16 translation, and the production of EGFP-p16 reporter bearing the miR-24 target recognition sites.
Conclusions/Significance
Together, our results suggest that miR-24 represses the initiation and elongation phases of p16 translation.
引用总数
学术搜索中的文章
A Lal, HH Kim, K Abdelmohsen, Y Kuwano… - PloS one, 2008