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Simultaneous saccharification and fermentation (SSF) experiments involving water hyacinth (Eichhornia crassipes), an abundantly available renewable bioresource, using hydrolytic enzymes, and fermentative microbes were investigated. Water hyacinth containing 30.01 (%, w/w) cellulose, 44.49 (%, w/w) hemicellulose, and 20.04 (%, w/w) lignin was subjected to three different pretreatments, namely, wet oxidation, phosphoric acid (H₃PO₄)‐acetone, and ammonia fiber explosion (AFEX). Hydrolytic enzymes, namely, recombinant Clostridium thermocellum cellulase (GH5) and hemicellulase (GH43), Trichoderma reesei and Bacillus subtilis AS3 cellulases were employed separately for saccharification. Saccharomyces cerevisiae and Candida shehatae were used for fermentation. The AFEX pretreated 1% (w/v) water hyacinth along with recombinant cellulase (GH5)‐hemicellulase (GH43) consortium gave the …