作者
V Courgnaud, F Laure, A Brossard, C Bignozzi, A Goudeau, F Barin, Ch BRÉCHOT
发表日期
1991/3
期刊
AIDS research and human retroviruses
卷号
7
期号
3
页码范围
337-341
简介
We have investigated in utero human immunodeficiency virus type 1 (HIV-1) transmission by analyzing human fetal tissues for the presence of viral DNA by means of the polymerase chain reaction (PCR). Thirty three fetal samples: thymus, spleen, and peripheral mononuclear blood cells (PMBC) were obtained at abortion (16 to 24 weeks) from HIV-1-infected asymptomatic women. The results of HIV-1 DNA detection were considered only in 9 cases where contamination of fetal samples by infected mother cells could be definitely eliminated by using primers specific for a polymorphic cellular locus. PCR allowed the identification of HIV-1 DNA sequences in 6/8, 8/9, and 5/9 of specimens from thymus, spleen, and PMBC, respectively. Positive results were shown in fetuses as early as 16 weeks. Viral cultures as well as assays for serum p24 HIV-1 antigen were negative in 9.9 and 33/33 tested, respectively. Therefore …
引用总数
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V Courgnaud, F Laure, A Brossard, C Bignozzi… - AIDS research and human retroviruses, 1991