作者
Wolfgang Bermel, Ivano Bertini, Isabella C Felli, Leonardo Gonnelli, Wiktor Koźmiński, Alessandro Piai, Roberta Pierattelli, Jan Stanek
发表日期
2012/8
期刊
Journal of biomolecular NMR
卷号
53
页码范围
293-301
出版商
Springer Netherlands
简介
The characterization of intrinsically disordered proteins (IDPs) by NMR spectroscopy is made difficult by the extensive spectral overlaps. To overcome the intrinsic low-resolution of the spectra the introduction of high-dimensionality experiments is essential. We present here a set of high-resolution experiments based on direct 13C-detection which proved useful in the assignment of α-synuclein, a paradigmatic IDP. In particular, we describe the implementation of 4D HCBCACON, HCCCON, HCBCANCO, 4/5D HNCACON and HNCANCO and 3/4D HCANCACO experiments, specifically tailored for spin system identification and backbone resonances sequential assignment. The use of non-uniform-sampling in the indirect dimension and of the H-flip approach to achieve longitudinal relaxation enhancement rendered the experiments very practical.
引用总数
20122013201420152016201720182019202020212022202320241141515112842142
学术搜索中的文章
W Bermel, I Bertini, IC Felli, L Gonnelli, W Koźmiński… - Journal of biomolecular NMR, 2012