作者
C Dika, JFL Duval, HM Ly-Chatain, C Merlin, CJAEM Gantzer
发表日期
2011/7/15
期刊
Applied and environmental microbiology
卷号
77
期号
14
页码范围
4939-4948
出版商
American Society for Microbiology
简介
We compare for the first time the electrokinetic and aggregation properties of MS2 phage (pH 2.5 to 7, 1 to 100 mM NaNO3 electrolyte concentration) with those of the corresponding virus-like particles (VLPs), which lack entirely the inner viral RNA component. In line with our previous work (J. Langlet, F. Gaboriaud, C. Gantzer, and J. F. L. Duval, Biophys. J. 94:3293-3312, 2008), it is found that modifying the content of RNA within the virus leads to very distinct electrohydrodynamic and aggregation profiles for MS2 and MS2 VLPs. Under the given pH and concentration conditions, MS2 VLPs exhibit electrophoretic mobility larger in magnitude than that of MS2, and both have similar isoelectric point (IEP) values (∼4). The electrokinetic results reflect a greater permeability of MS2 VLPs to electroosmotic flow, developed within/around these soft particles during their migration under the action of the applied electrical field …
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C Dika, JFL Duval, HM Ly-Chatain, C Merlin, C Gantzer - Applied and environmental microbiology, 2011