作者
Manuel Kratzke, Ermes Candiello, Bernhard Schmidt, Olaf Jahn, Peter Schu
发表日期
2015/8
期刊
Molecular neurobiology
卷号
52
页码范围
142-161
出版商
Springer US
简介
Adaptor protein (AP)-1/σ1B−/− mice have reduced synaptic-vesicle (SV) recycling and increased endosomes. Mutant mice have impaired spatial memory, and σ1B-deficient humans have a severe mental retardation. In order to define these σ1B−/− ‘bulk’ endosomes and to determine their functions in SV recycling, we developed a protocol to separate them from the majority of the neuronal endosomes. The σ1B−/− ‘bulk’ endosomes proved to be classic early endosomes with an increase in the phospholipid phosphatidylinositol 3-phosphate (PI-3-P), which recruits proteins mediating protein sorting out of early endosomes into different routes. σ1B deficiency induced alterations in the endosomal proteome reveals two major functions: SV protein storage and sorting into endolysosomes. Alternative endosomal recycling pathways are not up-regulated, but certain SV proteins are misrouted. Tetraspanins are …
学术搜索中的文章
M Kratzke, E Candiello, B Schmidt, O Jahn, P Schu - Molecular neurobiology, 2015