查看文章

Chronic liver diseases are a major cause of death globally. Hepatic stellate cells (HSC) will produce most of the excessive scar-tissue that disrupts the liver function. Although HSCs have been extensively investigated in the past decades, we do not know if their activation process from quiescent HSC to a scar-tissue producing cell is similar in different liver disease settings. In this project, we pool recently generated single-cell RNA-sequencing (scRNA-Seq) datasets containing HSCs in different injury settings to investigate what ligands and transcription factors drive HSC activation in different injury settings. 10 different mouse scRNA-Seq datasets, representing 7 different liver injuries, were integrated to create the mouse HSC activation atlas. Similarly, 3 different human scRNA-Seq datasets were integrated consisting of varying chronic liver diseases. Comparison of ligands and …